Dyah Rini Indriyanti, - and Sri Wahyuni, - and Priyantini Widiyaningrum, FMIPA Biologi and Ning Setiati, - (2020) Effect of Metarhizium anisopliae in Kaolin Formulation and Its Secondary Metabolite on Oryctes rhinoceros Larval Mortality. International Journal of Scientific & Technology Research, 9 (1). ISSN 2277-8616

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Abstract

Pest control of O. rhinoceros can be done using M. anisopliae entomopathogenic fungi. The purpose of this study was to analyze the effectiveness of the M. anisopliae in the kaolin formulation and secondary metabolite solution (MS) of M. anisopliae on the mortality of O. rhinoceros larvae. The population in this study was the O.rhinoceros larvae in from Jerukwangi Village, Bangsri District, Jepara Regency, Indonesia. The samples used were 30 instar 4 of O. rhinoceros larvae with a body weight of 13-16 g. This study used a Completely Randomized Design (CRD), which consisted of three groups with 10 replications. The groups consisted of control group, M group (2 g M. anisopliae in kaolin formulation), and MS group (25 ml of secondary metabolite solution in manure media). Observations were conducted every 2 days for 18 days. The results showed that the O. rhinoceros larvae in M. anisopliae group died in a hardened body state and the body was covered with green fungus hyphae. O. rhinoceros larvae in MS group died in a soft body state and were not overgrown with fungal hyphae on the surface of the larval body. M. anisopliae in kaolin formula kills O. rhinoceros larvae faster that the secondary metabolite solution. Larvae began to die on the 6th day after application and total death (100%) occured on the 18th day after the application. The secondary metabolite solution started to kill O. rhinoceros larvae on the 14th after application (20%) and on 18th day after the application, the death of O. rhinoceros larvae remained 20%.

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