Nugrahaningsih, WH Effect of Growth Regulators on Cell Growth and Flavonoid Production in Cell Culture of Elaecarpus grandiflorus. Conf. Series: Earth and Environmental Science 391 "Annual Conference on Environmental Science, Society and its Application ". pp. 1-7. ISSN 9781713802594

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Abstract

Abstract. Elaecarpus grandiflorus has the potential to be developed as a source of bioactive compounds. This study aims to obtain the most optimal medium for cell culture induction and flavonoid production in Elaeocarpus grandiflorus culture. Picloram (3.5, 5 and 7.5 ppm) and 2,4-D (1.5, 2.5 and 3.5 ppm) were used for induction of cell suspension culture. Cell suspension culture induction was observed through growth parameters (fresh and dry weight of cells) and cell suspension formation. In addition, it was found the production of flavonoids. Induction of cell culture is done by growing callus on Woody Plant Medium (WPM) with a variety of growth regulators. The culture was maintained in a shaker at a speed of 120 rpm for 30 days. At harvest, cells were filtered, weighed and dried. The spectrophotometer was used to determine the total flavonoid content. Quercetin was used as a standard compound. The best cell culture induction was obtained in cells maintained in WPM medium with the addition of 2,4-D 2,5 ppm. All cells in various treatment media can produce flavonoids with varying concentrations. WPM medium with the addition of 2,4 D and Picloram can be used for the production of flavonoids from Elaecarpus grandiflorus cells. Keywords: Elaecarpus grandiflorus, cell culture, 2,4D, picloram, flavonoid production

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