Niken Subekti, MIPA Biologi and Asni Puraedah, - and Dyah Rini Indriyanti, - and Agoes Soegianto, - (2020) Larvicidal and pupicidal activities from Citrus hystrix against Aedes aegypti mosquitoes. Ecology, Environtment and Conservation, 26 (3). pp. 1313-1318. ISSN 0971-765X

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Abstract

Aedes aegypti is a dengue vector that is known to be very threatening to human’s life. Dengue virus can cause dengue haemorrhagic fever (DHF) disease that are known to kill can human beings. Control is done during this using abate. However, abate is synthetic insecticide that is not environmentally friendly. Therefore, the control of mosquitoes by using natural materials such as essential oil of Citrus hystrixis one of the wise choice to mosquito control. This study aims to analyse the content of compounds in the essential oil of Citrus hystrix and analyse the mortality of larvae and pupa due to the provision of essential oil of kaffir lime based on nano emulsion technology. Essential oil is produced through the process of extracting Citrus hystrix using steam distillation, to determine the chemical compound of kaffir lime peel oil GC-MS was used, to make nano emulsionsonicator was used. Nano emulsion is then tested on larvae and pupa of Aedes aegypti. The identified major compounds were beta-pinene and limonene. The particle size identified from nano emulsion was ± 129.45 nm and ± 109.15 nm. The highest larvicidal activity was 21.6 ± 1.67% (LC50 = 546.592 ppm) and pupicidal activity 36 ± 1.67% (LC50 = 3045.323 ppm). Nano emulsion of essential oil of Citrus hystrix showed larvicidal and pupicidal activity on larva and pupa of Aedes aegypti and it is more environmentally friendly than abate. The results of this study can be recommended for control systems on Aedes aegypti mosquitoes

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