Senda Kartika, Rakainsa (2021) ISOLASI DAN ELUSIDASI STRUKTUR SENYAWA DARI EKSTRAK BAWANG DAYAK SERTA UJI AKTIVITAS ANTIBAKTERINYA. Jurnal Ilmiah Farmasi Imelda (JIFI), 4 (2). pp. 43-50. ISSN 2655-3147

Preview

PDF Download (581kB) | Preview

Abstract

Antibacterial activity screening was carried out on 10 species of medicinal plants that have been empirically used in traditional medicine in Indonesia, including the bulb of dayak onion (Eleutherine bulbosa), ki tolod herb (Isotoma longiflora), fruit of ketepeng cina (Cassia alata), and bluntas leaves (Pluchea indica). Based on the results of antibacterial activity screening, the extract of dayak onion (Eleutherine bulbosa) showed the most prominent antibacterial activity against Bacillus subtilis and Staphylococcus aureus at extract concentrations of 200 and 100 μg / ml, and against Klebsiella pneumoniae at an extract concentration of 200 μg/ ml. Purification and isolation of compounds and antibacterial activity assay were carried out to determine which compounds were responsible for the antibacterial activity of dayak onion. From the purification and isolation of the bioactive compound using MPLC, column chromatography, and preparative TLC, an isolate compound (4 mg) was isolated. The compound was analyzed using LR-EIMS and 1D-2D NMR, and was subsequently identified as Hongconin. The antibacterial activity test of dayak onion extract and isolated compounds showed that the extract of dayak onion exhibited moderate antibacterial activity against B. subtilis, S. aureus, and K. pneumoniae, indicated by the MIC value ranging from 100-200 μg/ml. Meanwhile, the isolate compound (Hongconin) exerted antibacterial activity which is classified as weak against all types of pathogenic bacteria tested, indicated by the MIC value of >200 μM.

Actions (login required)

View Item