Astrilia Damayanti, Teknik Kimia Unnes and Sarto, - and Wahyudi Budi Sediawan, - (2018) Kinetic Study of Limonene and Glucose Adsorption on Immobilization and Co-immobilization Beads. Reaktor, 18 (2). pp. 57-62. ISSN 0852 – 0798

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Abstract

Rotten oranges contain glucose and limonene, which is an inhibitor of microorganisms. Immobilization of mixed culture using entrapment method is the easiest method to protect the mixed culture from inhibitors. Entrapment method with extrusion drip is an efficient and effective technique to produce beads. This study aims to determine the adsorption rate of adsorbate (glucose and limonene) on the adsorbent surface (beads). Materials used in this study were glucose, DL-limonene, mixed culture, and beads. Three types of beads consisted of alginate-no mixed culture (A), alginate and activated carbon-no mixed culture (CA), alginate and activated carbon-free mixed culture (CB). Adsorption column consist of 30 ml nutrient, 15 mL substrate, and 5 mL beads. If the beads do not contain mixed culture, nutrients and substrate were replaced by distilled water, the reactor was done in a batch system at 37oC. The order of glucose adsorption capabilities starting from the lowest were AG, CAG, and CBG, while to limonene adsorption were AL, CBL, and CAL. Lagergren model was used to determined kinetic biosorption on limonene and glucose. The adsorption rate of the glucose in the pseudo-second order (k2,ad) ranged between 0.025 to 0.087 min-1, while the limonene ranged between 2.084 to 5.233 min-1. Adsorption of glucose and limonene on three types of adsorbent surfaces reached the steady state at the 60th minute.

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