Rengga, Wara Dyah Pita and Wulansarie, Ria and Putri, Echa Cahya Julyta Putri and Rustamadji, Rustamadji PENURUNAN BAKTERI VIBRIO SP PADA AIR TAMBAK UDANG VANAME (LITOPENAEUS VANNAMEI) MENGGUNAKAN TEKNOLOGI OZON. PENURUNAN BAKTERI VIBRIO SP PADA AIR TAMBAK UDANG VANAME (LITOPENAEUS VANNAMEI) MENGGUNAKAN TEKNOLOGI OZON. (Submitted)

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Abstract

One variety of shrimp, L.Vanamei, often uses brackish water during the operation in the shrimp pond. Chlorination and ultraviolet are usually used for disinfection of brackish water. However, it is ineffective and forms sediment in the water distribution. It can be a negative impact on the water quality cause a contamination on the shrimp, so the farmers might have loss of profit because Vibrio sp causes infection and dead on the shrimp. It affects the safety of consumers and should be minimized. The purpose of this study is to reduce the number of Vibrio sp bacteria in the pond water. The water was put in the storage tanks then pumped to filter out the impurities of the water. Furthermore, the water set the flow rate in 1 LPM, 2 LPM, and 3 LPM. After that, the ozone was injected to the water flow to sterilize the V. vulnificus bacteria. Finally, the water was returned to the original tank. The water from the tank was taken through a valve and analyzed in 0, 3, 7, 12, 18, 24, 30 minutes. The sample was analyzed immediately using a Total Plate Count method to determine the number of Vibrio sp bacteria in the shrimp pond water. The flow rate shows that the longer time of ozone made a lower amount of Vibrio sp bacteria. In 2 LPM water, it shows the optimum results of Vibrio sp bacteria reduction for 88.1% compared to the flow rate of 1 LPM and 3 LPM with the bacteria reduction of 68,8% and 70.6%. This study shows that the ozone with a flow rate of 2 LPM circulation is the most effective method to help reducing the number of Vibrio sp in brackish water distribution system in the shrimp environment and potentially as a disinfectant. Keywords: Flow rate; Ozone; Shrimp pond water; Vibrio sp

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