Megawati, Megawati and zuhriyan ash shiddieqy, bahlawan and Astrilia Damayanti, Teknik Kimia Unnes and Radenrara Dewi Artanti Putri, - and Bayu Triwibowo, - and Haniif Prasetiawan, - and septian putra kusuma aji, teknik kimia unnes and adi prawisnu, teknik kimia unnes (2022) Bioethanol production from glucose obtained from enzymatic hydrolysis of Chlorella microalgae. Materials Today: Proceedings, 63. : S373-S378. ISSN 2214-7853

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Abstract

Chlorella microalgae are one of the microalgae that can potentially be used as a substrate in ethanol production due to their high carbohydrate content. However, carbohydrates content in microalgae cannot be directly fermented into ethanol and need to be firstly hydrolyzed into glucose. The aim of this study was to develop a combined process of enzymatic hydrolysis and fermentation for the production of bioethanol from the Chlorella. The greatest glucose yield in the enzymatic hydrolysis process reached up to 80.01% under the condition of 3% (v/v) glucoamylase added, medium pH of 5.0, and hydrolysis temperature of 80 �C for five hours. The highest glucose concentration obtained from hydrolysis of Chlorella biomass in this study was subsequently fermented anaerobically using yeast Saccharomyces cerevisiae. From the fermentation process, the maximum ethanol yield reached 94.21% and the ethanol concentration was 4.80 g/L at a medium pH of 5.0 and a yeast load of 0.5 g for 30 h. Hydrolysis using enzymes has proven to be effective in the pretreatment of polysaccharides from the Chlorella into glucose and is suggested to be used as a substrate to produce bioethanol. Overall, Chlorella has great potential to be developed into sustainable renewable energy. � 2022 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (https://creativecommons.org/licenses/bync-nd/4.0). Selection and peer-review under responsibility of the scie

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