Nugrahaningsih, WH Extract of cell culture Rejasa (Elaeocarpus grandiflorus) Decrease Blood Glucose Through Insulin Receptor Pathway. Journal of Biology & Biology Education.

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Abstract

Diabetes mellitus is a metabolic disease characterized by the high blood glucose levels. The high prevalence of Diabetes Mellitus needed an innovation in prevention, treatment and control of case. The aim of research was to explore the potency of Rejasa (Elaeocarpus grandiflorus) as an antidiabetic. The pretest and posttest control group design were conducted to 30 hyperglycemic Wistar rat. The rats were divided into 5 groups, that were negative control (K-), positive control (K+, given glibenclamide 0.072 mg/200 kgBW), and experimental groups that given E. glandiflorus cell extract 1 mg/kgBW (P1), 10 mg/kgBW (P2), and 100 mg/kgBW (P3). The extract was given orally for 10 days. Measurement of blood glucose levels was carried out on day 0 and day 10. The mechanism of antidiabetic effect of E. glandiflorus was explored by in silico. The mean of blood glucose levels on day 0 were 455.2 mg/dL (K), 422.8 mg/dL (K+), 469.8 mg/dL (P1), 355.5 mg/dL (P2) and 446 mg/dL (P3). The blood glucose levels on day 10 were 367.8 mg/dL (K-), 89.6 mg/dL (K+), 285.6 mg/dL (P1), 136.8 mg/dL (P2) and 104.8 (P3). Statistical analysis showed the difference between K- from P2(p=0.015) and P3 (p<0.001). When compared with K+, only P3 showed no difference (p=0.873). Flavonoid of E. glandiflorus act on insulin receptor pathway and involved HK2, PTPN1, AKT1, PI3KR1, HRAS and GSK3B protein. The conclusion and the new finding of research that extract cell of E. glandiflorus have antidiabetic activity through insulin receptor pathway.

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