Ning Setiati, FMIPA Biologi and Partaya, - and N Hidayah, - (2020) The Use Of Two Pairs Primer For CO1 Gene Amplification On Traded Stingray At Fish AuctionTasik Agung Rembang. In: 6th International Conference on Mathematics, Science, and Education (ICMSE 2019), Unnes Semarang.

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Abstract

Stingray provide a high economic contribution and increase in production and utilization. Conservation effort needed to identify molecularly using CO1 gene. The success of CO1 gene in identifying species can not be separated from amplification in polymerase chain reaction (PCR) technique. The use of primer FishF1, FishR1, FishF2, and FishR2 to amplify COI gene for stingray traded at fish auction Tasik Agung Rembang never been done, so it need to be researched. The purpose of this study is to amplify the COI gene of stingray traded at fish auction Tasik Agung Rembang using two pairs of primers. Five stingrays sample were obtained from fish auction Tasik Agung Rembang. Then its DNA was isolated using protocol from TIANamp Marine Animals DNA Kit and measured in quantity using nano spectrophotometer. Amplification of CO1 gene by PCR technique. Based on the results of the study showed that the five samples had good DNA purity, concentration, and quality. The 5 samples amplification result showed that the FishF1-FishR1 primer pair succeeded in amplifying samples a, b, d, and e, while the FishF2-FishR2 primer pair succeeded in amplifying samples b, c, d, and e with a PCR product length around 655 bp

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