Habibah, Noor Aini (2021) Artikel Konservasi Tanaman Anggrek Gramatophyllum Secara In Vitro Melalui Pertumbuhan Minimal Menggunakan Paclobutrazol. Working Paper. Universitas Negeri Semarang.

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Abstract

The objective of this research was to study the minimum growth medium for Grammatophylum plantlet and the plantlets regeneration after grown in paclobutrazol medium at least in four months. The study was conducted experimentally using a complete random design. The independent variable in this study was concentrations of paclobutrazol added into the planting medium : 1, 3, and 5 mg/l. The dependent variable was the growth of shoot and the observed parameter of shoot growth was the number of leaves and shoot length after maintained in the medium with the addition of paclobutrazol in various concentrations for four months. The results showed that the growth of orchids on the medium with the addition of paclobutrazol treatment at all concentrations (1, 3, and 5 mg/l) had growth inhibition both in the number of leaves and shoot length. Growth inhibition that occured was varied depend on the addition of paclobutrazol concentration. Paclobutrazol treatment on Grammatophyllum plantlets was reduced the shoot length until 33.3% compared to the control at concentration of 1 mg/l. The number of leaves was also reduced until 29% compared to the control group at concentration of 5 mg/l paclobutrazol. These results indicated that paclobutrazol could be used as the short-term conservation of Grammatophyllum plantlets at 1-5 mg/l concentration. The conservation of Grammatophyllum using paclobutrazol should not exceed more than four months because it will reduce the quality of plantlets.

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