Priyantini Widiyaningrum, - and Niken Subekti, MIPA Biologi and Bambang Priyono, - (2019) Ecological characterization of local entomopathogenic nematodes Steinernema sp. and its pathogenicity test against termites Macrotermes sp. Songklanakarin J. Sci. Technol., 41 (2). pp. 459-464. ISSN 0125-3395

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Abstract

The evaluation of the ecological characteristics of local entomopathogenic nematodes (EPNs) Steinernema sp. using one-way classification experimental design was performed in this study. Infective juveniles (IJs) were maintained by in vivo culture, and its viability test at six storage temperatures, nine levels of pH and six levels of humidity were determined. The best viability was further tested for its pathogenicity on Macrotermes sp. Pathogenicity LC50 was calculated using Probit analysis. ANOVA was used to analyse the viability of IJs in all ecological parameters. The statistical analysis showed that viability of IJs was significant in all of the ecological parameters (LSD test; α < 0.05). The optimum viability of IJs was obtained at the temperature ranged from 21-27 °C ; pH 7.5-8.0 and moisture level of 80-100 %. Based on the Probit analysis, the pathogenicity LC50 was achieved at concentration 242 IJs/ml in 48 hours.

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