Sri Wardani, MIPA Pendidikan Kimia (2014) Inquiry Learning in Laboratory by HPLC Reversed-Phase Method Development in Taking the Conditions of Heavy Metals Separation. In: Proceedings of The 9 th Joint Conference on Chemistry, 12-13 November 2014, SEMARANG.

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Abstract

An analysis of chromatographic method for heavy metals has been developed through formation of complex compounds with di-n-buthyldithiocarbamate. Separation of the compounds was accomplished by HPLC reversed-phase using C18 column and eluent of methanol-water mixture with a composition of 70:30, at a flow rate of 0.75 ml/min and UV detection at 256 nm. DBDTC complexes ofCo (II), Ni (II), Cu (II), and Hg (II) were separable in less than 10 minutes, by injecting the complexes that had been added the ligand with concentration of 0,01 M. Based on their retention times, the sequence of polarity of the metal- DBDTC complexes, in a decreasing order, is as follows: Co (II) > Ni (II) > Cu (II) > Hg (II).

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