Study On The Effects Of Heavy Metals’ Pollution On The Activity Of 7-Ethoxyresorufin-O-Diethylase (EROD) In Two Freshwater Fish Species Of Indonesia

Nur Kusuma Dewi, BIOLOGI UNNES (2017) Study On The Effects Of Heavy Metals’ Pollution On The Activity Of 7-Ethoxyresorufin-O-Diethylase (EROD) In Two Freshwater Fish Species Of Indonesia. IInternational Journal of Scientific & Technology Research, 6 (6). 173-178 . ISSN 2277-8616

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Abstract

The study aimed to determine the effect of heavy metal pollution on 7-ethoxyresorufin-O-diethylase (EROD) enzyme activity in carp (Cyprinus carpio) and tilapia (Oreochromis niloticus). The samples were taken by purposive random sampling technique. The treatment site was the downstream part of the Kaligarang River, Semarang, Indonesia, which contaminated byheavy metals (concentration of Cd 0.007 ppm, Pb 0.010 ppm, and Hg 0.0006 ppm). A clean reference site in Nyatnyono Village, Ungaran was used as the control site with the heavy metal content of 0 ppm. The EROD activities in carp and tilapia were significantly decreased after Cd, Pb, and Hg exposure. The results showed that the average value of liver EROD activity in carp fish control group amounted to 1.77 ± 0.23µmol/min/mg protein and the treatment group was 0.49 ± 0.24µmol/min/mg protein. Whereas in control group of tilapia fish, EROD activity was equal to 2.08 ± 0.47µmol/min/mg protein and the treatment group was 0.49 ± 0.40µmol/min/mg protein. The results demonstrated that metallothionein was a specific heavy metals biomarker, whereas EROD activity ishighly sensitive to extremely low concentrations of theselected pollutants.In conclusion, the use of multiple biomarkers is recommended to monitor the heavy metal pollutants in the river environment.

Item Type:	Article
Uncontrolled Keywords:	EROD activity, heavy metals, biomarker, river environment
Subjects:	Q Science > QH Natural history > QH301 Biology
Fakultas:	Pasca Sarjana > Pendidikan IPA, S2
Depositing User:	mahargjo hapsoro adi
Date Deposited:	08 Sep 2019 13:20
Last Modified:	10 Sep 2019 23:59
URI:	http://lib.unnes.ac.id/id/eprint/32954

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