Rahayu, Enni Suwarsi and Habibah, Noor Aini IN VITRO SHOOT MULTIPLICATION OF Feronia limonia (L.) Swingle IN A CO ENRICHMENT AND SUCROSE REDUCTION CULTURE. In: INTERNATIONAL CONFERENCE ON CONSERVATION FOR BETTER LIFE, 11 - 13 September 2015, Semarang, Indonesia.

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Abstract

This present study was conducted to develop a non-conventional method of Feronia limonia (L.) Swingle shoots multiplication. The research was carried out experimentally using a completely randomized factorial design with two factors, i.e. sucrose concentrations of culture media (0%, 1%, 2%, and 3%); and CO levels of culture vessel (0.0%, ambient concentration, 0.03%, and 0.30%). Explants used in the research were cotyledonary nodes of sterile seedlings. Some variables observed include number of shoots produced in each explant, shoot height, internode length, and the number of composite leaves generated in each shoot after being cultured for three months. Data were analyzed in two-way Analysis of Variance and Duncan’s Multiple Range Test. Results showed that sucrose concentrations, CO 2 levels, and their interaction significantly influence the multiplication of F. limonia shoots. Murashige and Skoog medium containing 2% sucrose under 0.03% CO 2 2 level was the best treatment. It resulted in the highest number of shoots/explant, the tallest shoot, as well as relatively large amount of composite leaf and shoot. CO could replace a part of sucrose in the medium of F. limonia culture that used the cotyledonary node explants under red and blue light during the day and a temperature of 26 ± 2 °C.

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